right, I'm a bit puzzled. So the 12th fret harmonic is exactly an octave above the open string. and it's theoretically impossible for it not to be. and it's easier to tune the 12th fret harmonics, but when i tune the guitar that way, it doesnt sound very well in tune at all, so I then have to retune the open strings to eachother, making the harmonics out, but overall the guitar sounds good. So that leads me to believe that my 12th fret harmonics are not in fact in tune with the open string. testing it out directly, I see that they are only the same when i pick the open string absolutely as gently as I possibly can, so it's barely reading on the tuner. Any harder and the open string is sharp of the harmonic. So I see that pick strength on the note pitch has even more effect than I realised - should this be the case? cos it seems that if I want to SOUND in tune, I have to be technically out of tune. So then intonation comes into it. If i do the intonation with the 12th fret harmonic, or by playing the open string incredibly lightly, the intonation will surely still SOUND out? So what is a discerning guitarist to do? is there some way to minimalise the pitch change of a normal pick? Or should I simply do the intonation to make the 12th fret the same as a normal pluck of the open string instead of the harmonic? I guess guitar setup is all about compromise huh? Would a "better" guitar than my MIM standard strat reduce the problem somehow? if so what might be the difference? cheers!